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PRAS40 contributes to the tumorigenesis of CRC. (A) The AKT1S1 mRNA levels in COAD and READ samples from GTEx and TCGA. (B) The AKT1S1 mRNA levels in CRC samples from GSE32323 . (C) Kaplan-Meier analysis of RFS based on AKT1S1 mRNA levels in TCGA CRC patients. (D) Construction strategy and genotyping results of Akt1s1 +/+ and Akt1s1 -/- mice. (E-J) CRC mice model establishment <t>with</t> <t>AOM/DSS.</t> Experiment flow charts (E), representative images (F), quantitation of tumor number (G) and size (H), HE staining (I), western blotting analyses (J) and PCR analysis of Akt1s1 +/+ and Akt1s1 -/- mice (K). Scale bars, 500 μm. Data represent the mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001.
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PRAS40 contributes to the tumorigenesis of CRC. (A) The AKT1S1 mRNA levels in COAD and READ samples from GTEx and TCGA. (B) The AKT1S1 mRNA levels in CRC samples from GSE32323 . (C) Kaplan-Meier analysis of RFS based on AKT1S1 mRNA levels in TCGA CRC patients. (D) Construction strategy and genotyping results of Akt1s1 +/+ and Akt1s1 -/- mice. (E-J) CRC mice model establishment <t>with</t> <t>AOM/DSS.</t> Experiment flow charts (E), representative images (F), quantitation of tumor number (G) and size (H), HE staining (I), western blotting analyses (J) and PCR analysis of Akt1s1 +/+ and Akt1s1 -/- mice (K). Scale bars, 500 μm. Data represent the mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001.
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PRAS40 contributes to the tumorigenesis of CRC. (A) The AKT1S1 mRNA levels in COAD and READ samples from GTEx and TCGA. (B) The AKT1S1 mRNA levels in CRC samples from GSE32323 . (C) Kaplan-Meier analysis of RFS based on AKT1S1 mRNA levels in TCGA CRC patients. (D) Construction strategy and genotyping results of Akt1s1 +/+ and Akt1s1 -/- mice. (E-J) CRC mice model establishment with AOM/DSS. Experiment flow charts (E), representative images (F), quantitation of tumor number (G) and size (H), HE staining (I), western blotting analyses (J) and PCR analysis of Akt1s1 +/+ and Akt1s1 -/- mice (K). Scale bars, 500 μm. Data represent the mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001.

Journal: Neoplasia (New York, N.Y.)

Article Title: PRAS40 activates the IRE1α-XBP-1-mediated unfolded protein response to exacerbate colorectal cancer by enhancing ST6Gal1-dependent α-2, 6 sialylation of GRP78

doi: 10.1016/j.neo.2026.101297

Figure Lengend Snippet: PRAS40 contributes to the tumorigenesis of CRC. (A) The AKT1S1 mRNA levels in COAD and READ samples from GTEx and TCGA. (B) The AKT1S1 mRNA levels in CRC samples from GSE32323 . (C) Kaplan-Meier analysis of RFS based on AKT1S1 mRNA levels in TCGA CRC patients. (D) Construction strategy and genotyping results of Akt1s1 +/+ and Akt1s1 -/- mice. (E-J) CRC mice model establishment with AOM/DSS. Experiment flow charts (E), representative images (F), quantitation of tumor number (G) and size (H), HE staining (I), western blotting analyses (J) and PCR analysis of Akt1s1 +/+ and Akt1s1 -/- mice (K). Scale bars, 500 μm. Data represent the mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Azoxymethane (AOM) and polybrene was from Sigma-Aldrich; dextran sodium sulfate (DSS) was from MP Biomedicals; PNGase F was purchased from Takara Bio Inc. (Dalian, China); β-sitosterol ( ≥ 98%) was purchased from YuanYe Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Quantitation Assay, Staining, Western Blot